Enzymes and Inhibitors
Invitrogen™ Ambion™ DNase I (RNase-free)
Nonspecific endonuclease that degrades double- and single-stranded DNA and chromatin
Thermo Scientific™ FastDigest DpnI
Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI).
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Thermo Scientific™ Reaction Buffer with MgCl2 for DNase I (10X)
Optimize reaction condions for RNase-free DNase I with this 10X reaction Tris-HCl (pH 7.5) buffer containing MgCl2.
Thermo Scientific™ EcoRI (10 U/μL)
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ FastDigest Esp3I (IIs class)
Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest KpnI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Exonuclease I (20 U/μL)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Thermo Scientific™ FastDigest NdeI
The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).
Thermo Scientific™ FastDigest HindIII
The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ HindIII (10 U/μL)
The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.
Thermo Scientific™ FastDigest BpiI (IIs class)
Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).